Serodiagnosis of Louse-Borne relapsing fever with glycerophosphodiester phosphodiesterase (GlpQ) from Borrelia recurrentis
Fri, 2007-02-02 15:01 — Vince Smith
| Publication Type | Journal Article | |
| Year of Publication | 2000 | |
| Authors | Porcella, S. F.; Raffel, S. J.; Schrumpf, M. E.; Schriefer, M. E.; Dennis, D. T.; Schwan, T. G. | |
| Journal Title | Journal of clinical microbiology | |
| Volume | 38 | |
| Issue | 10 | |
| Pages | 3561-3571 | |
| Journal Date | Oct | |
| Accession Number | 44975 | |
| Key Words | Amino Acids; Animals ; Bacterial Proteins/analysis/genetics ; Borrelia ; Cloning; Molecular ; DNA ; Ethiopia ; Fluorescent Antibody Technique; Indirect ; Humans ; Insect vectors ; Lice/microbiology ; Phylogeny ; polymerase ; sequence ; Serologic Tests | |
| Abstract | Human louse-borne relapsing fever occurs in sporadic outbreaks in central and eastern Africa that are characterized by significant morbidity and mortality. Isolates of the causative agent, Borrelia recurrentis, were obtained from the blood of four patients during a recent epidemic of the disease in southern Sudan. The glpQ gene, encoding glycerophosphodiester phosphodiesterase, from these isolates was sequenced and compared with the glpQ sequences obtained from other relapsing-fever spirochetes. Previously we showed that GlpQ of Borrelia hermsii is an immunogenic protein with utility as a serological test antigen for discriminating tick-borne relapsing fever from Lyme disease. In the present work, we cloned and expressed the glpQ gene from B. recurrentis and used recombinant GlpQ in serological tests. Acute- and convalescent-phase serum samples obtained from 42 patients with louse-borne relapsing fever were tested with an indirect immunofluorescence assay (IFA) and an enzyme-linked immunosorbent assay (ELISA) that used whole cells of B. recurrentis and with immunoblotting to whole-cell lysates of the spirochete and Escherichia coli producing recombinant GlpQ. The geometric mean titers of the acute- and convalescent-phase serum samples measured by IFA were 1:83 and 1:575, respectively. The immunoblot analysis identified a high level of reactivity and seroconversion to GlpQ, and the assay was more sensitive than the whole-cell IFA and ELISA using purified, recombinant histidine-tagged GlpQ. Serum antibodies to GlpQ and other antigens persisted for 27 years in one patient. We conclude that assessment of anti-GlpQ antibodies will allow serological confirmation of louse-borne relapsing fever and determination of disease prevalence. | |
| Notes | LR: 20041117; JID: 7505564; 0 (Bacterial Proteins); 0 (DNA Primers); 0 (DNA, Bacterial); EC 3.1.4 (Phosphoric Diester Hydrolases); EC 3.1.4.46 (glpQ protein, Bacteria); ppublish0095-1137 Journal | |
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